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DR.WU粉餅 驗出有毒重金屬鋇(Barium)
charles201309 在天空部落發表於10:50:31 | Life 人生
DR.WU粉餅 驗出有毒重金屬鋇(Barium
20140416 
蘋果日報
(方翊倩/綜合報導)
DR.WU粉餅,驗出有毒重金屬鋇。翻攝《壹週刊》
DR.WU粉餅,驗出有毒重金屬鋇。翻攝《壹週刊》
DR.WU登記的土城工廠地址,未見門牌號碼。翻攝《壹週刊》
DR.WU粉餅,驗出有毒重金屬鋇。翻攝《壹週刊》
DR.WU粉餅,驗出有毒重金屬鋇。翻攝《壹週刊》
 
《壹週刊》抽驗美妝龍頭DR.WU旗下產品,意外發現其中一項粉餅產品檢驗出有毒重金屬鋇(Barium),其數值還比環保署公告的污水排放容許值高出6倍。
今日出刊的《壹週刊》報導,《壹週刊》在北市新光三越A8館購買DR.WU旗下產品,包括「礦質無暇雙效粉餅(自然色)」和「角鯊潤澤修護精華」,送交SGS(台灣檢驗科技股份有限公司)進行常見的八大重金屬檢驗。結果礦質無暇雙效粉餅(自然色)驗出鋇。
327日再度購買DR.WU礦質無暇雙效粉餅(自然色)和礦質無暇雙效粉餅(白皙色)送驗。結果礦質無暇雙效粉餅(自然色)鋇含量高達6.76ppm,白皙色粉餅鋇含量是5.12ppm
醫師表示,重金屬鋇具有心臟、神經毒性,吸入或誤食後,會產生心律不整和血壓升高的症狀,嚴重會造成呼吸衰竭,以及傷害肝臟、腎臟功能,如果皮膚接觸到鋇,也會有刺激性,輕則發癢,重則潰爛。
DR.WU天昱生物科技公司田姓公關表示,礦質無暇雙效粉餅是委託韓國製造,他們沒有添加這個成分,為何會有鋇?可能是原料天然礦物晶石有鋇所致,公司事先有把關送驗。

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1985年4月16日台灣第一個試管嬰兒在台北榮總醫院出生 國內第一個試管嬰兒張先生現在29歲正在念動物學博士學位
charles201309 在天空部落發表於09:26:27 | Cell Engineering 細胞工程
1985416日台灣第一個試管嬰兒在台北榮總醫院出生 國內第一個試管嬰兒張先生現在29歲正在念動物學博士學位
20140416
莫忘來時路/中國時報 黃如萍
29年前的今天,台灣第一個試管嬰兒在台北榮總醫院出生(張昇平提供),為不孕夫婦帶來希望。
 
29年前的今天,台灣第一個試管嬰兒在台北榮總醫院出生,為不孕夫婦帶來希望。
所謂試管嬰兒是由人工操作,將卵子和精子取出後體外受精,並培養成胚胎,26天後再將胚胎植回母體內,利用體外受精技術所生出來的嬰兒。
世界第一個試管嬰兒是1978年在英國誕生,台灣晚了7年,香港則是到1986年、大陸1988年才有本土試管嬰兒誕生。目前台灣有40多個不孕症診所或中心,每年約70008000個試管嬰兒出生,全球約有3百萬名試管嬰兒。
試管嬰兒成功機率大約35%,台灣和美國差不多;國際上最著名的就是一對以色列夫婦,前後做了8次試管嬰兒才順利懷孕。
為了增加成功機率,試管嬰兒常會植入多胞胎,考量母體與孩子教養,台灣限制不得超過4胞胎;多胞胎的成功機率也和歐美相同,約25%30%。台灣的紀錄保持人是連續3次進行試管嬰兒,成功產下一胞胎、三胞胎及雙胞胎,33男共6個小孩。
世界第一個試管嬰兒布朗小姐,已結婚並育有兒子,是上班族國內第一個試管嬰兒張先生,現在29歲,正在念動物學博士學位。醫學專家證實,試管嬰兒在健康上和一般人無異,人格與發展成就端視家庭教育與養成,以及個人的努力
試管嬰兒的誕生證明了醫學技術可以彌補不孕夫婦的遺憾,但有人擔心,未來隨著基因診斷、重組等技術的純熟,試管嬰兒的體外授精技術將被更精進的利用,未來訂做一個有諾貝爾獎得主頭腦、名模漂亮臉蛋與身材或NBA球星壯碩體格的下一代,不是夢想,遺傳、種族、倫理等概念將被顛覆,甚至連自我價值都得重新審視。試管嬰兒將造成文化、倫理與道德的爭議。

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英國東倫敦大學21歲男子毀容 手術重現俊臉 成模特兒 參加時裝秀
charles201309 在天空部落發表於08:42:15 | Life 人生
英國東倫敦大學21歲男子毀容 手術重現俊臉 成模特兒 參加時裝秀
20140416  
旺報即時 吳貴奉
羅賓斯整容前後對比照片。(摘自環球網)
 
模特兒一般對相貌要求很高,但英國一名曾經毀容的男子最後圓了自己的模特兒夢。據香港《東方日報》415日報導,英國東倫敦大學21歲男生羅賓斯樣貌俊朗,且獲模特兒公司相中,準備向模特兒工作發展。但沒想到後來因事故遭到暴打被毀容。
據環球網報導說,在20131221日晚,羅賓斯在科爾賈斯特一間酒吧外,見到一名女子遭6人圍住。他上前為女子解圍時,反遭圍毆,導致鼻骨、頰骨和眼窩被打傷。
羅賓斯在醫院看見自己樣貌被毀,以為模特兒夢已粉碎。但經過4個多月的治療,醫生為他進行臉部重整手術,包括固定鼻骨和在頰骨植入金屬片,羅賓斯最後重現俊臉。雖然他的左臉還有少許腫,和鼻子有疤痕,但他於2周前終得償宿願,首次踏上「天橋」,參加了時裝秀。

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國健署:肥胖、少運動、飲食不健康 三大新興致癌因子
charles201309 在天空部落發表於08:35:23 | Life 人生
國健署:肥胖、少運動、飲食不健康 三大新興致癌因子
20140416
中國時報 洪欣慈/台北報導
國健署指出,不健康的飲食、肥胖率高及運動量少是台灣人癌症的3項新型殺手。圖為民眾在休閒中心運動的畫面。(本報資料照片)
3大新興致癌因子
 
台灣癌症人數居高不下,致癌因子除了已知的菸酒、檳榔外,不健康的飲食、肥胖及運動量少,更是癌症的3項新型殺手。
國健署指出,肥胖者罹患乳癌、子宮內膜癌、結直腸癌等疾病的危險性皆較常人高12倍;身體運動量不足,也與乳癌、大腸癌息息相關。
國健署長邱淑媞表示,根據FAO(世界農糧組織)統計,台灣在肉類及油脂性食物的可獲性都高過日本、韓國等鄰近亞洲國家,顯示肉類和油脂性食物在國人飲食結構中相當重要。
愛吃肉卻少吃菜、少運動,國健署統計顯示,國內有150019歲以上成年人每日蔬果攝取量未達建議標準;男女缺乏運動的比率分別達64.4%73.1%,與OECD(經濟合作暨發展組織)其他30個國家相比,各排名第二及居冠。
中研院生物醫學研究所研究員潘文涵表示,肥胖、運動不足、不健康飲食三者息息相關,吃不對卻又不運動,等同雪上加霜;肥胖之所以成為新興致癌因子,原因在於肥胖細胞會分泌賀爾蒙、促進癌症發展,也會讓身體發炎指數升高、降低免疫力
她進一步說,飲食不當與各種癌症也都有關係,像食道癌與飲食過燙有關,大腸直腸癌則可能是攝取過量油脂,蔬果攝取量不足也與許多癌症密切相關。

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Seven Million Grads Transform China's Workforce in High-Tech Threat to U.S.
charles201309 在天空部落發表於08:07:13 | Life 人生
 

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Awakened by Cellular Stress:Isolation and Characterization of a Novel Population of Pluripotent Stem Cells Derived from Human Adipose Tissue(3)
charles201309 在天空部落發表於09:52:39 | Cell Engineering 細胞工程
Awakened by Cellular StressIsolation and Characterization of a Novel Population of Pluripotent Stem Cells Derived from Human Adipose Tissue3
http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0064752
Results
Muse-ATs Isolated from Lipoaspirated Human Adipose Tissue
Adipose tissue is composed of adipocytes (mature cells) and the stromal vascular fraction (SVF) containing a heterogeneous population of cells, including adipose tissue macrophages (ATMs), adipose stem cells (ASCs), mesenchymal stem cells, and fibroblasts.
We explored the possibility of both activating and isolating Muse-AT cells from their quiescent state by exposing them to cellular stress (Fig. 1A). Lipoaspirated material was first incubated in collagenase for 30 min at 37°C to release adipocytes (floating cells) and different cellular components present in the SVF as previously described. This material was then subjected to severe cellular stress, including long incubation with collagenase, low temperatures, low serum and hypoxia, to kill fragile adipose cells and release Muse-AT cells. Optimal conditions for the release of Muse-AT cells were determined to be 16 hours incubation with collagenase in DMEM medium without FCS at 4°C under very low O2, which subsequently gave way to a homogenous population of Muse-AT cells. Approximately 90% of isolated cells were both SSEA3 and CD105 positive, as determined by flow cytometry (Fig. 1B). This high purity is presumably due to the severity of the cellular stress conditions, responsible for the depletion of other cell types. As all other components of the adipose tissue lipoaspirate failed to survive, a population of highly purified Muse-AT cells was obtained, and therefore further purification processes were not necessary. Muse-AT cells were plated in both adherent and non-adherent cell culture dishes. We observed that Muse-AT cells can grow either in suspension or in adherence culture to form the characteristic cell clusters observed in ES cell-derived embryoid body, as described in bone marrow and dermal fibroblast-derived Muse cells in previous reports (Fig. 1C, D). Under both conditions, individual Muse-AT cells reached a diameter of around 10µm and cell clusters reached a diameter of up to 50µm by day 3 (Fig. 1C–D), which has been previously demonstrated to mark the limit of their proliferative capacity.
 
Figure 1. Isolation and morphologic characterization of Muse-ATs.
(A) Schematic of Muse-AT isolation and activation from their quiescent state by exposure to cellular stress. Muse-AT cells were obtained after 16 hours, with incubation with collagenase in DMEM medium without FCS at 4°C under very low O2 (See Methods). (B) FACS analysis demonstrates that 90% of isolated cells are both SSEA3 and CD105 positive. (C) Muse-AT cells can grow in suspension, forming spheres or cell clusters as well as individual cells (see red arrows) or (D) Muse-AT cells can adhere to the dish and form cell aggregates. Under both conditions, individual Muse-AT cells reached a diameter of approximately 10µm and cell clusters reached a diameter of up to 50µm, correlating to stem cell proliferative size capacity.
doi:10.1371/journal.pone.0064752.g001
 
Muse-ATs Spontaneously Express Pluripotent Stem Cell Markers
Upon transfer and adherence to chamber slides for immunofluorescent staining, both the Muse-AT cell clusters and individual Muse-AT cells strongly expressed all of the characteristic pluripotent stem cell markers that were examined. These included SSEA3, a cell-surface glycosphingolipid frequently used to detect human ES cells and to purify Muse cells from bone marrow and dermis; Oct3/4 a protein involved in the self-renewal of human ES cells; Nanog, a transcription factor involved in the self-renewal of human ES cells; Sox2, a transcription factor that controls genes involved in embryonic development; and TRA-1-60, which reacts with the antigen TRA-1-60 on the surface of embryonic germ cells and ES cells (Fig. 2). Comparatively, ASCs derived from the same lipoaspirated tissue were either negative or weakly positive for these pluripotent stem cell markers (Fig. 2).
 
Figure 2. Muse-ATs express pluripotent stem cell markers.
Immunofluorescence microscopy demonstrates that Muse-AT aggregates, along with individual Muse-AT cells, express characteristic pluripotent stem cell markers, including SSEA3, Oct3/4, Nanog, Sox2, and TRA1-60. Comparatively, ASCs (right panel) derived from the same lipoaspirate under standard conditions (see above, were negative for these pluripotent stem cell markers. Nuclei were stained with DAPI (blue). Original magnification, 600 X.
doi:10.1371/journal.pone.0064752.g002

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台積電法說 陸行之提四問
charles201309 在天空部落發表於09:16:33 | Life 人生
台積電法說 陸行之提四問
鉅亨網新聞中心 來源:聯合報系/udndata.com 2014-04-14
 
台積電法說會本周四登場,挑起敲響台股2014年首季法說行情重任,成為法人觀察半導體產業景氣、甚至台股後續走勢的重要指標。外資圈包括巴克萊、麥格理率先上調台積電目標價,對營運展望投下肯定票。
巴克萊亞太區半導體產業首席分析師陸行之延續慣例,在法說會前端出「四問台積電」。分別是:
一、至明年下半年前,台積電如何規畫20奈米與16奈米的產能?
二、20奈米、16奈米量產除可推升營收,台積電認為對整體毛利率影響為何?
三、台積電未來二年如何看待指紋感測器、心跳感測、智慧手表、智慧眼鏡等產品的營收貢獻?
四、台積電對2014年營收成長預估值是否有上調至二成的機會?
 
陸行之說,台積電不僅首季可繳出每股純益上看1.75元的成績單,展望第2季,受惠蘋果、高通、聯發科等大廠需求,台積電的28奈米、20奈米金屬閘極(HKMG)製程將放量,第2季營收季增率可達14%18%,產能利用率也會來到100%水準。
就個別公司競爭力分析,德意志證券半導體產業分析師周立中認為,因二線晶圓廠的良率相對較低,會驅使客戶積極向台積電下單,預料一直到年底前,台積電2820奈米等製程產能都將十分吃緊。半導體一線大廠獨霸局面,儼然成形。
麥格理證券亞太科技產業研究部主管蘇志凱對台積電第2季營運展望則更加樂觀。麥格理估計,台積電第2季將交出營收季增二成成績單,更引人注目的是,從本季起至2015年底,台積電每一季的每股純益都將突破2元。換言之,半導體龍頭的營運榮景,現在還只是開端,未來會更亮麗。
【記者簡威瑟、魏興中/台北報導】

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保有適量脂肪 才能維持身體健康 不要過分在意體重 反而維持適量活動、規律又均衡的飲食方式 才是保持健康的最好方法
charles201309 在天空部落發表於09:09:49 | Bariatrics 肥胖醫學
保有適量脂肪 才能維持身體健康 不要過分在意體重 反而維持適量活動、規律又均衡的飲食方式 才是保持健康的最好方法
作者:【記者陳敬哲/綜合外電報導】 | 台灣新生報 – 2014415
 
健康是否與瘦直接劃上等號,可能要重新思考。學術期刊Journal of Epidemiology中提出,不到平均年紀死亡的民眾,70%體重過輕,人數遠大於身體肥胖者;然而社會風氣不斷將瘦與健康劃上等號,讓許多人不斷節食害怕體重上升,但身體保有適量脂肪,才能維持健康
美國心臟科醫師Carl Lavie提出,健康身體絕對不是瘦,而是保持良好的脂肪與肌肉比例,體重絕對不是魔鬼,某些方面,身體需要脂肪才能打敗疾病,但現在激進的社揮風氣,認為體脂肪是萬惡來源,不斷壓低身體質量指數,盡可能讓身體脂肪量趨近於零,可是這絕對不是健康之道,反而造成傷害。
民眾絕對不要過於擔心體脂肪,美國一名61歲男性,20年前曾經心臟病發,目前體重約86公斤,雖然體重超標,但醫生建議不要過分在意體重,反而維持適量活動、規律又均衡的飲食方式,才是保持健康的最好方法Carl Lavie提醒,許多美式足球運動員,雖然體重超出標準,但不代表不健康
身體質量指數能夠計算脂肪比率,超過25達到超重,超過30達到肥胖,但Carl Lavie提醒,體脂肪絕對不是惡夢,體脂肪比率超重的民眾,其實不需要過份擔心,只要每天保持活動,營養均衡的飲食方式,不會因多出標準體重10公斤導致傷害,仍然可以很健康維持生活。

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太陽花學運中的柄谷行人(からたに こうじん、Kojin Karatani)影子
charles201309 在天空部落發表於09:03:48 | Life 人生
太陽花學運中的柄谷行人(からたに こうじん、Kojin Karatani)影子
20140415 04:10
張瑞昌專欄-中國時報 張瑞昌
太陽花學運期間,就讀台大政研所的學運總指揮林飛帆,被媒體捕捉到懷裡揣著一本《柄谷行人談政治》(心靈工坊出版)的畫面,引起外界的關注議論。(左,本報資料照片)
 
柄谷行人(からたに こうじんKojin Karatani)何許人也?何以成為學運領袖的思想導師?
1941年出生的柄谷行人,擁有多重身分,他既是文學評論家,也是左翼批判理論家,更是革命行動的實踐者。早於1960年風起雲湧的安保鬥爭中,當時就讀東京大學經濟學部的柄谷,即投入「全學連」而成為安保世代的一員。
研究領域跨越哲學、經濟、政治及社會的柄谷行人,已被視為當代日本頗具分量的思想家,他曾先後受邀在耶魯、哥倫比亞、康乃爾及加州大學洛杉磯分校等美國名校擔任客座教授,重要著作有《倫理21》、《近代日本文學之起源》、《超越的批判康德與馬克思》、《邁向世界共和國》及《世界史的構造》等。
 
《柄谷行人談政治》學運衝向武裝 定被消滅
《柄谷行人談政治》一書是以訪談紀錄的形式,從60年代安保鬥爭與全共鬥運動的觀察,一路談到他如何走上思想家之路,還有他對歷史與反覆的思辨、對自由主義與新自由主義的反省,乃至對國家資本主義、日本代議制度的批判,以及九一一之後的世局解析。
學運領袖是如何從這本書取經,我無從知悉,不過最新一期的亞洲周刊訪問了《柄谷行人談政治》的譯者台南藝術大學音樂系主任林暉鈞,談到一些有意思的觀察心得。比方說,他認為,日本60年代的學運曾歷經兩個不同階段,1960年的第一次安保鬥爭,學運只是整個社運的一部分,但到了1968年第二次安保鬥爭,工、農皆已停擺,僅剩學運苦撐,最終走向激進路線,因而失去中產階級的支持
「我想林飛帆了解這一點,所以他一再強調和平、非暴力,但和平、非暴力會有它的限制,有點兩難。」林暉鈞如此說道,柄谷當年的學運經驗值得借鏡之處,「就是不要衝太遠、不要太快,不要衝到武裝去,那一定會被消滅。」
這是學者試著觀察學運與書中經驗的對比,柄谷還提出許多有趣的論述,像「昭和是明治的反覆」,藉以說明世界資本主義的周期循環長度約60(更長的是120年周期),簡單說,即1930年歷史發生的事件會在1990年重演,他以此理論解釋近衛文磨與其外孫細川護熙兩位前首相,在明治與昭和兩段歷史舞台的相對應。
 
示威是民主主義的支柱
又如他評論日本代議制已成貴族政治,有力政客皆是政治二世、三世,甚至四世的權貴勢力,這樣的代議制,「就是選舉出代表者的寡頭政治,這絕對稱不上是民眾參與的民主。」因此,柄谷說,「如果只憑藉代議制度,不可能改變民主主義。實際上即使在美國,也有很多示威活動;他們的選舉本身就帶有示威的性質。示威這種行為,正是民主主義的支柱。」
然而,也許柄谷的書的確指引了太陽花學運,但書中沒有提及的是,帶頭從事社會運動不可能不付出代價,尤其這個代價往往是改變一生的重大轉折。
當年的安保鬥爭,擔任東京大學全共鬥議長的山本義隆是物理系博士生,他被視為天才學者,未來角逐諾貝爾物理獎的熱門人選,結果他遭警方逮捕入獄,儘管出獄後仍勤於筆耕,不少物理教科書也皆出自他的手,但未完成學業的山本,只是一名大學講師,在福島核災後書寫著述,人們若還有印象,也不過就是電影《革命青春》中K一心想假冒扮演的那個學運領袖罷了。
二次安保中另一個足以和山本齊名的是日本大學全共鬥議長秋田明大,這位經濟系高材生率眾在校內構築防禦工事,與警方展開激烈戰鬥,最後雖然贏得勝利,卻引來政府介入,警察機動隊進入校園,而他也難逃入獄的命運。現在67歲的秋田,在故鄉廣島經營一家修車廠,幾年前他離婚後透過婚姻介紹所再婚,娶了一個年輕20歲的中國女人安度餘年。
柄谷有許多前進的理論,譬如他鼓吹個人成立小型共同體對抗國家和財團,研判「網路可能幫助示威活動的組織與集結」;又如他分析,10萬人的街頭抗議不會對政府構成威脅,真正對政府造成威脅的是,這種行為背後大量人民的反對意見
反覆閱讀咀嚼,書中訪談的內容彷彿似曾相識,我想這應該不是事後諸葛吧!

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強辯拒妥協 馬黨政崩盤 全民總統 與民作對 幾乎確定提前「跛鴨」 遑論追求「歷史定位」
charles201309 在天空部落發表於08:49:07 | Life 人生
強辯拒妥協 馬黨政崩盤 全民總統 與民作對 幾乎確定提前「跛鴨」 遑論追求「歷史定位」
20140415 04:10
中國時報 楊毅/新聞分析
黨國分崩離析
 
學運退場後,綠營迅速以「世代傳承」改革回應。反觀執政的國民黨,至今卻依然故我,未見有任何深切反省,裝作好像一切都沒發生過似的。不僅「馬王政爭」再起,郝胡朱吳連等中生代或地方諸侯,更是作壁上觀,黨內分裂危機隱隱浮現。「後馬時期」各方勢力接班卡位蠢蠢欲動、暗潮洶湧,是一大警訊。
 
民怨爆發 仍拒傾聽
順利連任總統、黨魁,馬英九黨政權力「一把抓」,以黨輔政、黨政密合的結果,卻是執政聲望跌落谷底,民調支持度僅剩個位數,創下另類「台灣奇蹟」。執政黨的灰頭土臉,孰令致之?
回顧馬英九執政6年多來,歷經美牛案、油電雙漲、復徵證所稅、12年國教等治理危機,黨內領導地位和執政威望,早已危如累卵,更讓社會累積、凝聚龐大「反馬」能量。
 
全民總統 與民作對
一向標榜公平正義的馬英九,從大埔拆遷、核四存廢到洪仲丘案等,卻不斷引爆社會反彈聲浪。不僅如此,學貸沉重壓力,低薪資、高房價等問題,更讓年輕人幾乎看不見未來希望,充滿焦慮感,背後牽涉世代剝奪、世代正義等嚴肅課題,終在此次學運「總爆發」。
面對民怨沸騰,馬英九選擇的往往不是放下身段,謙虛傾聽、溝通或對話,反而是凡事都要爭個輸贏,都要搞清是非曲直,沒有任何妥協、包容空間
表面上宣稱要當「全民總統」,實際上卻是處處和全民「作對」的總統,口惠而實不至。
去年9月政爭爆發後,馬英九堅持「大是大非」,不惜讓國會空轉,國政延宕,總統和國會議長對簿公堂,早已埋下憲政危機的導火線。
學生占領國會,只不過是點燃引信,讓「馬王心結」爆發,成為「壓倒駱駝的最後一根稻草」,服貿過關遙遙無期,馬英九可說是「輸到脫褲」了。
 
內外交迫 提前跛鴨
學運退潮後,未來將轉守為攻、遍地開花,各式各樣抗爭運動,勢必益發風起雲湧,馬政府無法維護民主法治的後果就是,未來馬走到哪,黑潮就會跟到哪,如影隨形。
不僅如此,郝龍斌、朱立倫等黨內中生代的合縱連橫;丁守中、連勝文為了角逐首都市長,殺得刀刀見骨,都嚴重挑戰馬的黨內領導地位。
此刻的馬英九內外交迫、腹背受敵,幾乎已確定提前面臨「跛鴨」危機,更遑論追求什麼「歷史定位」了。
一場太陽花學運,突顯出政府施政無能、執政黨失能的問題與荒謬,國民黨這家「百年老店」,若再繼續自我感覺良好,無視問題癥結,不趕緊振衰起弊、急起直追,失去政權和民心,只是遲早的事!

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人才瘋西進 搶高薪賺資歷 人才外流是國安問題
charles201309 在天空部落發表於08:42:49 | Life 人生
人才瘋西進 搶高薪賺資歷 人才外流是國安問題
【經濟日報記者陳致畬、張運祥/專題報導】   2014.04.15
圖/經濟日報提供
圖/經濟日報提供
 
台灣薪資零成長,大陸薪資連續十年兩位數增長,造成台灣人才在大陸職場性價比提高,結果是愈來愈多的大陸企業願意僱用台灣人,前往大陸工作的台灣人也有年輕化趨勢
電子商務帶來一波人才革命,網路金融顛覆傳統金融的創新獲利模式,催化台灣電商和金融人才外流大陸。十年前銀行業就有不少人才進入大陸銀行,協助對方發展信用卡業務;傳道授業的大學教授也早已絡繹於途,站上大陸高校舞台。
大陸市場大,楚材晉用,為了成就,也為薪資加倍,讓夢想起飛,這一波人才外流來勢洶洶,去的多,回來的少
本報記者陳致畬、張運祥分赴珠三角和長三角的廣州、上海,前進大陸企業,採訪在陸企第一線奮戰的台灣人,為讀者提供第一手觀察報導。
半年前,大陸一家企業老闆找上人力資源專家黃至堯,請他幫忙找一位年薪750萬元的行銷總監;黃至堯在台灣找個半死,就是找不到合適人選。黃至堯說:「台灣的問題出在工資太低,工資低就會變得沒機會。」
黃至堯是一勢人力資源研究中心的負責人,是個獵頭專家;每年經過他手中完成的獵才案件,總薪酬超過新台幣1億元以上。他的客戶開玩笑說,「不找黃至堯,當然找不到人。」
終究還是有黃至堯找不到的人,他說,台灣沒有領年薪750萬元的行銷總監;後來,他找了一個年薪350萬元的人給大陸老闆,對方居然不屑一顧。
 
大陸老闆 看的是格局
大陸老闆對黃至堯說,我一年的行銷預算是人民幣1億元,相當新台幣5億元,請一個年薪350萬元的人,不到人民幣100萬元,他有這個能力嗎?他會不貪汙嗎?
「大陸老闆說的沒錯,」黃至堯說,他們在意的是「格局」,5億元的預算計畫,對一個過去只做過1,000萬元預算的人來說,怎麼做得出50倍的計畫來?
商業發展研究院商業發展與政策所所長杜震華表示,「台灣薪資低廉,如果我從美國念完博士,也不想回來台灣服務,肯定去更高薪的香港、新加坡等地發展」。
「政府已警覺人才外流是國安問題,」杜震華說,台灣最具影響力的研究機構中研院經濟所,職缺已經多年沒人來應徵;政府應盡速拿出具體解決辦法,否則人才外流只會更加嚴重。
 
人才流失 成國安問題
台灣人才在大陸性價比高,人才外流大陸是個趨勢1111人力銀行最新調查,國內高達95%的上班族有意赴大陸發展,登陸就業意願比一年半前調查的77%高出很多。
1111人力銀行公關總監李大華說,台灣青年(1524歲)失業率高達12.66%,顯示青年就業的困境,越年輕的族群西進意願越高,反應出台灣嚴峻的就業環境,已成為對青年人才外移的推力。
這幾年各國人才湧進大陸一線城市,上海、北京房價和房租只漲不回,建築設計是直接受惠的行業,問題是這個行業也找不到人來做。有些台商被迫回台再設一家公司,把大陸的工作拿回台灣來做,這已變成一種趨勢。
「建築設計缺人,只好把上海公司的案子拿回台灣來消化。」上海奇顯建築設計公司總經理沈凱說,台灣設計人才到大陸工作,薪水是台灣的1.52一、二年後,大陸公司就以多出三到四成的薪資挖角;台灣員工發現他是從一個小池子游到大海裡,於是就用海的條件跟台商談薪資,最後很多台灣企業就死在海上。
他感慨地說,陸企財大氣粗,台商把人才帶到大陸去,都是在幫大陸企業獵才和培訓。「人才流動就像做貿易一樣,低買高賣,」黃至堯說,建築設計人才大學畢業薪水23萬元,做個三、五年後,也不過45萬元,到大陸去就翻倍了。
現在台灣大學生海外留學,同學都是大陸人,學成後第一站是直接去大陸就業,根本沒想過回台灣工作。廣州旅遊度假集團人力資源部培訓總監呂秀如表示,有機會還是想回台灣貢獻所學,問題是台灣能給出多少機會?
2014/04/15 經濟日報】

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Awakened by Cellular Stress:Isolation and Characterization of a Novel Population of Pluripotent Stem Cells Derived from Human Adipose Tissue(2)
charles201309 在天空部落發表於18:12:23 | Cell Engineering 細胞工程
Awakened by Cellular StressIsolation and Characterization of a Novel Population of Pluripotent Stem Cells Derived from Human Adipose Tissue2
Methods
Isolation of Muse-AT cells from Lipoaspirated Fat
Lipoaspirates (100–200 g per aspirate) were obtained from subcutaneous abdominal adipose of women undergoing elective liposuction. None of the investigators of this study had any contact with, nor any knowledge of any personal information relating to, these patients. Furthermore, human subjects were unidentifiable as well as all their characteristics and clinical records. Therefore, this study did not meet the criteria of human subjects research and HHS regulations did not apply (45 CFR 46.102(f)).
Lipoaspirate was repeatedly Washed with PBS until blood was completely removed from the tissue, and then incubated with equal volume of DMEM containing collagenase (0.1%, Sigma Aldrich) for 30 min at 37°C in a shaking incubator at 110 rpm, followed by incubation in 4°C, while still in collagenase and nutritionally deficient medium (no FCS), for 16 hours under severe hypoxia conditions. Digested material was then centrifuged at 1500 rpm for 10 minutes at 4°C. Supernatant containing adipose cell debris (dead adipocytes, macrophages, red blood cells, adipose stem cells among other cell components) was removed by aspiration and the remaining cell Pellets were washed several times with PBS. Pellets were re-suspended in PBS and incubated with a red blood cell lysis buffer (eBiosciences, San Diego, CA) for 10 min at R/T (2×). Remaining cell pellets containing cells highly resistant to severe cellular stress, were re-suspended in Dulbecco’s Modified Eagle Medium 1× (DMEM; CellGro, MediatechInc, Manassas, VA) comprised of 10% fetal bovine serum (FBS; Thermo Scientific Hyclone, Logan, UT) and 5% antibiotic-antimyocotic solution (CellGro, Mediatech Inc, Manassas, VA), and plated as cells in suspension as well as adherent cells. For ASC isolation, lipoaspirate material was subjected to collagenase digestion (0.1%, Sigma Aldrich) for 30 min at 37°C in a shaking incubator at 110 rpm, and ASCs were isolated and cultured as previously described.
 
Flow Cytometry Analysis
Floating Muse-AT cells were cultured in DMEM/10% FCS for 2 days followed by FACS analysis. Cells were washed in 2% inactivate FCS/0.05% sodium Azide/PBS and were re-suspended in 100 µl of the same buffer and incubated at 4°C for 1 hour in the presence or absence of primary unconjugated rat anti-human SSEA3 (EMD Millipore; Billerica, Massachusetts). Cells were then washed twice with the same buffer and incubated with the corresponding secondary FITC-conjugated anti-rat IgM (BD Biosciences; San Diego, CA) for 45 minutes at 4°C. After two consecutive washes, cells were incubated with PE-mouse anti-human CD105 (BD Biosciences, San Diego, CA) at 4°C for 1 hour. Cells were then washed and re-suspended in 200 µl of the same buffer. Analysis of count and cell type was performed using a FACS Calibur flow cytometer and cEllQuest Pro software.
 
Immunocytochemistry
Cells were fixed in 4% paraformaldehyde (20 min at R/T), washed in PBS, then incubated in 0.2% Triton for 20 min. After 2 successive washes in PBS, cells were blocked with 10% normal goat serum in 1% BSA solution for 60 min at R/T. Cells were then incubated with the primary antibodies overnight at 4°C. The following pluripotent stem cell markers were used: rat anti-human stage-specific embryonic antigen (SSEA3, Millipore, Billerica, MA), mouse anti-human octamer-binding transcription factor 3 and 4 (Oct3/4, Santa Cruz Biotech, Santa Cruz, CA), rabbit anti-human Nanog (Millipore, Billerica, MA), rabbit anti-human SRY-box 2 (Sox2, Millipore, Billerica, MA), and mouse anti-human TRA-1-60 (Abcam, Cambridge, MA); for mesenchymal cell lineages: rabbit anti-human preadipocyte factor 1 (Pref-1, [a.k.a. delta-like 1 homolog (drosophila), DLK1] preadipocyte marker, Santa Cruz Biotech, Santa Cruz, CA); mouse anti-human myosin D (MyoD, myocyte marker, R&D Systems, Minneapolis, MN), and mouse anti-human smooth muscle actin (SMA, myocyte marker, Thermo Scientific, Waltham MA); for endodermal cell lineages: mouse anti-human pan keratin (Santa Cruz, CA); rabbit anti-human α-fetoprotein (Dako, Santa Clara, CA); and mouse anti-human cytokeratin 7 (Millipore, Billerica, MA); and for ectodermal cell lineages: mouse anti-human neuron specific enolase (NSE, Millipore, Billerica, MA); rabbit anti-human glutamate receptor (Abcam, Cambridge, MA); rabbit anti-human NeuroD (Chemicon, Temecula CA); mouse anti-human nestin (Chemicon, Temecula CA); and rabbit anti-human microtubule-associated protein 2 (MAP2, AbDSerotech, Raleigh, NC). All primary antibodies were diluted 1:200 in PBS/0.1% BSA solution. Following treatment with primary antibodies, cells were washed 3 times with PBS and incubated for 1 hour at R/T with PBS/0.1% BSA containing secondary immunofluorescent antibodies (1:1000) Alexa Fluor 488 conjugated dye (mouse or rat, Invitrogen, Carlsbad, CA) or Texas Red conjugated dye (rabbit, Invitrogen, Carlsbad, CA). Cells were washed 4X with PBS and treated with PBS/0.2% DAPI for 10 minutes. Cells were then washed 3X with PBS. Images were acquired with an Evos immunofluorescence inverted microscope (Advanced Microscopy, Mill Creek, WA).
 
Induced Differentiation of Muse-ATs
Various differentiation media were used to induce differentiation of Muse cells-AT to the three germline cell lineages. For adipocyte formation, adherent Muse-AT cells were treated with adipogenic differentiation medium containing DMEM with 0.5 mM isobutylmethylxanthine, 1 µM dexamethasone, 10 µM insulin, 200 µM indomethacin and PPAR-γ (ZenBio, Inc, Research Triangle Park, NC) over 3 or 6 days at 37°C and 5% CO2. Adipocytes were detected using fluorescence lipid drop marker BODIPY-C16 (1:1000, Invitrogen, Carslbad, CA) following manufacturer specification.
For myocyte formation, adherent Muse-AT cells were incubated in DMEM containing with 10% FBS, 5% NHS, 50µM hydrocortisone, and 1% antibiotic-antimycotic solution over 3 or 6 days at 37°C and 5% CO2. Smooth muscle cells were identified by expression of smooth muscle actin (SMA) and skeletal muscle cells myosin D.
For hepatocyte and biliary cell induction, adherent Muse-AT cells were incubated in hepatocyte differentiation medium for 3 or 6 days, as previously described adherent Muse-AT cells were incubated in DMEM supplemented with 10% FBS, 10 µg/ml insulin, 5.5 µg/ml transferring, 6.7 ng/ml sodium selenite (ITS; Gibco, Life Technologies, Grand Island, NY), 10 nM dexamethasone (Sigma-Aldrich, St. Louis, MO), 100 ng/ml hepatocyte growth factor (HGF, Peprotech, Rocky Hill, NJ) and 50 ng/ml and fibroblast growth factor- 4 (FGF-4, R & D Systems, Minneapolis, MN) for 3 or 6 days. Hepatocytes were identified by immunohistochemistry using cytokeratin 7 and α-fetoprotein expression.
For neural cell formation, Muse cells-AT were incubated as non-adherent cells in ultra-low attachment plates (Corning Incorporated, Life Sciences, Manassas, VA) in the presence of neural differentiation medium 1 containing Neurobasal medium (Gibco, Life Technology, Grand Island, NY) supplemented with B-27 supplement serum free (Gibco, Life Technology, Grand Island, NY), 100 µg/ml kanamycin (Gibco, Life Technology, Grand Island, NY), 2 mM glutamine (Sigma-Aldrich, St. Louis, MO), 30 ng/ml bFGF (Peprotech, Rocky Hill, NJ) and 30 ng/ml EGF (Peprotech, Rocky Hill, NJ) for 7 days. Cells were then transferred to polystyrene culture slides (BD Biosciences, San Jose, CA) and cultured for another 7 days as adherent cells in the presence of neural differentiation medium 2 containing 1 DMEM supplemented with 2% FCS, 25 ng/ml bFGF and 25 ng/ml BDNF (Peprotech, Rocky Hill, NJ). Neural cells were identified by immunohistochemistry using nestin and MAP2 as indicated above.
 
Microarray Analysis
Muse-AT cells and ASCs were isolated from lipoaspirate material of three different patients. RNA was extracted using an RNeasy Mini Kit (Qiagen) and analyzed by Hokkaido System Science Co. Ltd. Array signals were processed and normalized using the GeneSpring GX version 12.1.0 (Agilent Technologies). Data has been deposited into the Gene Expression Omnibus databank with the access number GSE46353. The criteria for selecting differentially-expressed genes were preset as at least 2-fold difference in either direction plus statistical significance (P<0.05, unpaired t test). Microarray analysis was performed using the software program IPA via a license to Ingenuity (https://analysis.ingenuity.com/pa/login/login.jsp) to identify (1) functional pathways (cell function, physiological function, diseases), (2) canonical signaling pathways (3) networks of related genes derived from genes changed in the analyzed comparisons and (4) upstream regulators. Further information regarding gene function was obtained from the program GeneDecks V3 at www.genecards.org. Statistical analyses were carried out by Fischer’s exact test (as performed automatically by the software). In determining which genes are only expressed in either Muse-ATs or ASCs, all samples, having been performed in triplicate, had to display uniform detection (indicated with at least 100 standard units) or absence (at most 30 standard units) along with a P-value <0.05.

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Awakened by Cellular Stress:Isolation and Characterization of a Novel Population of Pluripotent Stem Cells Derived from Human Adipose Tissue(1)
charles201309 在天空部落發表於18:10:25 | Cell Engineering 細胞工程
Awakened by Cellular StressIsolation and Characterization of a Novel Population of Pluripotent Stem Cells Derived from Human Adipose Tissue1
Saleh Heneidi, Ariel A. Simerman, Erica Keller, Prapti Singh, Xinmin Li, Daniel A. Dumesic, Gregorio Chazenbalk   
PublishedJune 05, 2013
DOI10.1371/journal.pone.0064752
 
Abstract
Advances in stem cell therapy face major clinical limitations, particularly challenged by low rates of post-transplant cell survival. Hostile host factors of the engraftment microenvironment such as hypoxia, nutrition deprivation, pro-inflammatory cytokines, and reactive oxygen species can each contribute to unwanted differentiation or apoptosis. In this report, we describe the isolation and characterization of a new population of adipose tissue (AT) derived pluripotent stem cells, termed Multilineage Differentiating Stress-Enduring (Muse) Cells, which are isolated using severe cellular stress conditions, including long-term exposure to the proteolytic enzyme collagenase, serum deprivation, low temperatures and hypoxia. Under these conditions, a highly purified population of Muse-AT cells is isolated without the utilization of cell sorting methods. Muse-AT cells grow in suspension as cell spheres reminiscent of embryonic stem cell clusters. Muse-AT cells are positive for the Pluripotency markers SSEA3, TR-1-60, Oct3/4, Nanog and Sox2, and can spontaneously differentiate into Mesenchymal, endodermal and ectodermal cell lineages with an efficiency of 23%, 20% and 22%, respectively. When using specific differentiation media, differentiation efficiency is greatly enhanced in Muse-AT cells (82% for mesenchymal, 75% for endodermal and 78% for ectodermal). When compared to adipose stem cells (ASCs), microarray data indicate a substantial up-regulation of Sox2, Oct3/4, and Rex1. Muse-ATs also exhibit gene expression patterns associated with the down-regulation of genes involved in cell death and survival, embryonic development, DNA replication and repair, cell cycle and potential factors related to oncogenecity. Gene expression analysis indicates that Muse-ATs and ASCs are mesenchymal in origin; however, Muse-ATs also express numerous Lymphocytic and Hematopoietic genes, such as CCR1 and CXCL2, encoding chemokine receptors and ligands involved in stem cell homing. Being highly resistant to severe cellular stress, Muse-AT cells have the potential to make a critical impact on the field of regenerative medicine and cell-based therapy.
 
Citation: Heneidi S, Simerman AA, Keller E, Singh P, Li X, et al. (2013) Awakened by Cellular Stress: Isolation and Characterization of a Novel Population of Pluripotent Stem Cells Derived from Human Adipose Tissue. PLoS ONE 8(6): e64752. doi:10.1371/journal.pone.0064752
Editor: Alexander V. Ljubimov, Cedars-Sinai Medical Center, United States of America
Received: February 7, 2013; Accepted: April 17, 2013; Published: June 5, 2013
Copyright: © 2013 Heneidi et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: Part of these studies were supported by the Department of Obstetrics/Gynecology at University of California Los Angeles and by the Eunice Kennedy Shriver National Institute of Child Health & Human Development, National Institutes of Health through cooperative agreement U54 HD071836. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
 
Introduction
Cellular stress is induced by abrupt disruption of the physiological niche: the optimal home most conducive to cell survival. Although adult stem cells have been considered an attractive source for cell therapy, their effectiveness and efficiency is hindered by a frequently low survival rate due to their exposure to a high cellular stress environment upon transplantation. This key limitation is observed when utilizing adult stem cells for regenerative purposes, as typical cell engraftment yields are extremely low (<3%). Multiple factors contribute to this low rate of cell survival, including the harsh environment of the recipient site, harboring pro-apoptotic factors including hypoxia, malnutrition, pro-inflammatory cytokines and reactive oxygen and nitrogen species. The severity of cellular stress is heightened when stem cells are administered to an acutely injured area, such as a myocardial infarction, stroke, or a peripheral ischemic injury, as are the chances of unwanted activation or differentiation of surviving cells. It is extremely difficult to alter the environment of the damaged tissue, which necessitates a viable alternative: to improve post-transplant stem cell survival rates through the administration of a stem cell population with the adaptations necessary for survival in the hostile host environment.
One potential solution to this problem is to gradually adapt stem cells to cellular stress prior to cell delivery. It has been shown that introducing stem cells to hypoxic conditions in vitro for a duration of 24–48 hours, also known as Hypoxia preconditioning (HPC), provides the opportunity for these cells to adapt to low oxygen concentrations, thus increasing chances for survival upon reintroduction to hypoxic conditions in vivo. HPC is a promising solution to the severe apoptosis that accompanies transplantation as it induces an adaptive mechanism that increases the likelihood of cell survival in a pro-apoptotic microenvironment in vivo. Adult human Mesenchymal stem cells (MSCs) and adult Hematopoietic stem cells (HSCs) have similarly been shown to increase expansion, survival, and self-renewal under hypoxia conditions while maintaining the capability for multi-lineage differentiation.
Another potential solution to the problem of successful delivery of stem cells to a hostile host environment is to utilize a purified population of stem cells, isolated during exposure to severe cellular stress conditions (e.g. long time incubation to proteolytic enzymes, hypoxic conditions, serum deprivation, low temperatures), for engraftment. Recently, a new stem cell population has been isolated from mesenchymal tissues such as human skin fibroblasts and bone marrow stromal cells under cellular stress conditions. These cells, termed Multilineage Differentiating Stress-Enduring (Muse) Cells, are of Mesenchymal stem cell origin and comprise 1–3% of the entire cell population. Muse cells exhibit characteristics of both Mesenchymal and Pluripotent stem cells. They are double positive for CD105, a mesenchymal stem cell marker, and Stage specific embryonic antigen-3 (SSEA3), well known for the characterization of undifferentiated human embryonic stem cells (ES) from bone marrow aspirates or from cultured mesenchymal cells such as bone marrow stromal cells and dermal fibroblasts. They express Pluripotency markers including Oct3/4, Nanog and Sox2, differentiate into cells of ectodermal, endodermal, and mesodermal lineages both in vitro and in vivo, and have the ability to self-renew. Advantageously, Muse cells do not appear to undergo tumorigenic proliferation, and therefore would not be prone to produce teratomas in vivo, nor do they induce immuno-rejection in the host upon autologous transplantation. In addition, Muse cells are shown to home into the damage site in vivo and spontaneously differentiate into Tissue specific cells according to the Microenvironment to contribute to Tissue regeneration when infused into the blood stream. Therefore, they exhibit the potential to make critical contributions to tissue regeneration in the absence of restrictions attributed to the difficult extraction of bone marrow stromal cells and human skin fibroblasts, and time-consuming purification methods such as cell sorting. In order to increase the viability of Muse cells as a source of tissue regeneration, a more accessible supply must be utilized.
Harvesting human adipose tissue by Lipoaspiration is a safe and non-invasive procedure, and hundreds of millions of cells can be isolated from 1–2 liters of lipoaspirate material. Therefore, adipose tissue could prove the ideal source for Muse cell isolation as opposed to bone marrow or dermis. Using lipoaspirate material, we developed a novel methodology for the isolation of a population of human Muse cells under Severe cellular stress conditions (long term incubation with Proteolytic enzyme, 4°C, serum deprivation, and Hypoxia). Purification of human Muse cells derived from adipose tissue (Muse-ATs) does not require the use of cell sorting, magnetic beads or special devices. Muse-ATs can grow either in suspension, forming cell spheres, or as adherent cells forming cell aggregates similar to human ES cell-derived embryoid bodies as previously reported. Furthermore, Muse-AT cells express Pluripotent stem cell markers and a variety of markers indicative of all three germlines. Upon the introduction to specific culture conditions, Muse-AT cells can differentiate to mesenchymal (adipocytes, skeletal and smooth muscle cells), endodermal (hepatocytes and biliary ducts) and ectodermal (neural cells) cell lineages both spontaneously and by differentiation induction. Immunocytochemistry and microarray data demonstrate Up-regulation of the Pluripotent stem cell markers Sox2, Oct3/4, and Rex1 in Muse-AT cells, as compared to previously studied multipotent adipose stem cells (ASCs). Microarray analysis reveals that Muse-AT cells highly express genes involved in Cellular protection against Oxidative stress. Additionally, these cells also exhibit up regulation of CXCL2 gene expression, a critical Chemokine involved in stem cell Homing. Muse-AT cells display down regulation of genes involved in cell death and survival, embryonic development, organism survival, cellular assembly and organization, mitosis, DNA replication, recombination and repair. Because lipoaspiration is a safe and non-invasive procedure and Muse-AT cell isolation requires a simple yet highly efficient purification technique, Muse-AT cells could provide an ideal source of pluripotent-like stem cells with the potential to have a critical impact on regenerative medicine and cell-based therapy.

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Muse cell(Multi-lineage differentiating Stress Enduring cell)
charles201309 在天空部落發表於17:41:22 | Cell Engineering 細胞工程
Muse cellMulti-lineage differentiating Stress Enduring cell
Muse cellMulti-lineage differentiating Stress Enduring cellis a newly discovered non-tumorigenic pluripotent Stem cell.
Muse cells reside in mesenchymal tissues such as Bone marrow, Dermis and Adipose tissue as well as in commercially obtainable mesenchymal cellshuman fibroblasts and bone marrow. Muse cells can be obtained fromBone marrow aspirateAdipose tissue and liposuctionDermisCommercially available culture cells such asBone marrow-derived mesenchymal stem cellsFibroblastsAdipose-derived stem cells.
Muse cells are able to generate cells representative of all three germ layers from a single cell both spontaneously and under cytokine induction.
Muse cells do not undergo teratoma formation when transplanted into a host environment in vivo. This can be explained in part by their intrinsically low telomerase activity, eradicating the risk of tumorigenesis through unbridled cell proliferation.
 
Muse cell
  • Pluripotent stem cells, which can generate various kinds of the cells representative of all three germ layers have the ability to self-renew.
  • Non-tumorigenic.
  • Exhibit Tissue repair effect when supplied to the blood stream.
  • Can be collected from Bone marrow, Dermis, Adipose tissue and commercially available Fibroblasts.
  • Comprise ~0.03% of bone marrow transplantation and several % of mesenchymal stem cell transplantation.
  • Can be isolated as cells positive for SSEA-3, a well known human embryonic stem cell marker.
  • Pluripotent stem cells can be directly obtained from normal human mesenchymal tissues without using artificial manipulations such as gene introduction.
 
Muse cells are identified as cells which  
  • are positive for SSEA-3+, a well-known marker for undifferentiated human ES cells. Cell isolation by SSEA-3 cell sorting can be done using SSEA-3 antibody.
  • are positive for general mesenchymal stem cell markers such as CD105, CD90 and CD29.
  • are double positive for Pluripotent and Mesenchymal stem cell markers.
  • do not express CD34hematopoietic and adipose stem cell markersand CD117hematopoietic stem cells markers, Snai1 and Slugskin-derived precursors markers, CD271 and Sox10neural crest-derived stem cells markers, NG2 and CD146perivascular cellsor CD31 and von Willebrand factorendothelial progenitor markers. This indicates that Muse cells do not belong to previously investigated stem cell types.
 
Muse cell in regenerative medicine
  • Bone marrow transplantationMuse cells are a subpopulation of bone marrow cells. They represent a small population of mono-nucleated bone marrow cells(~0.03%. This means that they have already been supplied to patients many times all over the world in bone marrow transplantations; a well-known procedure that has been performed in clinics since 1958.
  • Mesenchymal stem cell transplantationMuse cells exist within cultured MSCs such as bone marrow mesenchymal stem cells and adipose-derived stem cells. MSC transplantation has been employed for repairing liver, heart, neural tissue, airway, skin, skeletal muscle, and intestine. Therefore, if Muse cells were purified or enriched, the effectiveness of currently performed MSC transplantation is expected to see vast improvements.
  • Because Muse cells do not form teratomas in vivo, they could provide an ideal source of Pluripotent stem cells for Regenerative medicine and Cell-based therapy.


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醫療資訊太難懂 半數美國人寧相信陰謀論
charles201309 在天空部落發表於09:32:13 | Life 人生
醫療資訊太難懂 半數美國人寧相信陰謀論
作者:中時電子報 – 2014413
中國時報
 
美國醫療技術雖然先進,但最新研究指出,約半數美國人相信至少1種毫無根據的醫療陰謀論,例如固定接種疫苗會造成自閉症之類。
芝加哥大學研究團隊主導此研究。201389月,研究人員請1,351名美國成人閱讀6個流行的醫療陰謀論,接著請受試者線上回答是否聽過或贊同這些陰謀論,最後按比率進行統計。
這些陰謀論包括,政府故意不讓民眾取得另類療法;政府明知道使用手機會致癌,卻置之不理;政府刻意利用基因改造有機體,來縮減全球人口數;固定接種疫苗會造成自閉症,政府知道卻不處理;飲用水所以加氟,是為了掩蓋業者用危險化學物汙染環境的事實等。
結果顯示,49%的受試者相信至少1種醫療陰謀論,37%的受試者徹底相信政府封鎖自然療法的管道。69%的受試者聽過接種疫苗會造成自閉症的說法,20%的人贊同,44%不同意。
唯一逾半數受試者都不認同的陰謀論是,「美國情報機構故意讓大批非裔美國人感染愛滋病毒」。
此研究主要作者、芝加哥大學政治學教授艾瑞克.奧利佛說,人們相信陰謀論,乃是因為陰謀論比複雜的醫療資訊好懂多了
研究人員並表示,對陰謀論深信不疑的病患,較可能尋求另類療法,而非循傳統醫學管道治療
奧利佛說,醫生不應把這些病患當成瘋子,而是必須瞭解他們可能較不會按照醫囑用藥。
他說:「對那些教育程度較低的民眾而言,拒絕以科學方式思考事情較為容易。」
他指出,增加大眾的健康與科學資訊至關重要。
此研究刊登於《美國醫學會內科醫學期刊》線上版。

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幹細胞治療效果 有待檢驗
charles201309 在天空部落發表於09:27:42 | Cell Engineering 細胞工程
幹細胞治療效果 有待檢驗
作者:洪欣慈台北報導 | 中時電子報 – 2014413
中國時報【洪欣慈台北報導】
 
幹細胞一直被脊髓損傷者、神經疾病患者視為治癒的一線曙光,數年前前總統陳水扁孫子出生時保留下來的臍帶血,也被寄予讓其夫人吳淑珍再站起來的厚望。醫師表示,幹細胞相關研究與臨床試驗一直在進行,但要證明療效,恐還有漫漫長路要走。
台大台成幹細胞治療中心主任唐季祿表示,周邊血液幹細胞是藉由施打白血球生長激素(G-CSF),將骨髓中的幹細胞驅動至血液中,再經由血液分離機收集取得,目前主要用於治療血癌等血液疾病,抽取技術已十分純熟
唐季祿說,過去國內外有許多研究,欲試驗周邊血液幹細胞對於帕金森氏症、中風等神經疾病的療效,但都還有爭議,原因在於中風病人經過一段時間,也會自己慢慢恢復機能,是否由幹細胞促成不得而知,國內外報告結果也不一。
他表示,幹細胞要運用於治療中風,效果最好的應是神經幹細胞,但因其需在體外培養、培養成功後再放回身體裡,需嚴格的體外培養條件,國內外對這部分的法規也較嚴格。 
台大神經部腦中風加護病房主任鄭建興表示,很多國家都使用不同來源的幹細胞來進行腦中風試驗,但成效可能會依急性、慢性腦中風的不同,或患者病變狀況而有所差異。這次林欣榮的試驗看起來有得到一些效果,但要推廣到一般治療,恐怕還有很大一段距離。
馬偕醫院癌症中心主任謝瑞坤表示,人體免疫系統比想像中複雜,加上要操控生長因子並非易事,這也是多數幹細胞研究尚無法直接運用於臨床的原因,初步臨床結果還需更廣泛試驗,才能確認療效。

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醫學新發現!移植自體周邊血液幹細胞改善腦中風失能
charles201309 在天空部落發表於09:21:52 | Cell Engineering 細胞工程
醫學新發現!移植自體周邊血液幹細胞改善腦中風失能
作者:華人健康網 記者洪毓琪/台北報導 | 華人健康網 – 2014412
中風不僅會手腳發麻,還有可能出現眩暈、嘔吐、頭痛、步態不穩等
 
中風之所以令人害怕,除了它經常突然發生,事先沒有明顯的預兆、即易導致猝死外,就是即使將中風患者從鬼門關救回來,通常會留下行動不便的後遺症,嚴重影響日後生活。不過,針對這樣的情形未來也許有治療、改善的機會。在台灣醫師將於國際期刊「細胞移植Cell Transplantation」發表的臨床試驗成果指出,移植自體周邊血液幹細胞,可有效改善中風患者的身體失能。
中國醫藥大學北港附設醫院暨安南醫院院長林欣榮表示,國內每年有10萬以上的中風新增病例,使得腦中風成為導致國人殘疾的主因。一般來說,當中風沒有早期發現,在發病6個月以上就進入慢性期,復健較難再有起色,這是因為腦部神經受損,一直缺乏有效療法來改善。
該項臨床試驗,是透過收集患者本身的周邊血液幹細胞,再利用腦部定位技術,將幹細胞植入腦中,透過幹細胞的修復、再生功能,讓患者中風受損的腦神經再生,恢復原本的功能。這項試驗相當成功,術後患者的各項腦中風評估指數,都有明顯提升。影像醫學也顯示,腦部受傷部位的神經有再生現象。試驗過程未見嚴重副作用,證實其安全無虞。
 
2期臨床試驗結果成功 進行第3期臨床試驗確認可行性
林欣榮醫師表示,目前完成的二期臨床試驗結果,已被國際知名期刊《細胞移植》(Cell Transplantation)所接受,將於近期內刊出。這是全球首度進行的嘗試,希望能為再生醫學帶來新思維。接下來團隊將展開第三期臨床試驗,進一步確認該療法的可行性。除此之外,林欣榮醫師還將與全球脊椎治療權威,美國紐澤西Rutgers大學教授楊詠威(Dr. Wise Young)一同合作,進行臍帶血幹細胞治療脊髓損傷的試驗。
 
【醫學小知識周邊血液幹細胞】
幹細胞只存在於骨隨之中?事實上,平常在骨髓內負責人體白血球,紅血球及血小板的製造幹細胞(Stem cell),在人類出生後,雖然主要分佈在人體的骨髓中,但其周邊血液中,仍有其少量的幹細胞存在,不過由於含量極低,在以往無法透過分離得到足夠幹細胞作為臨床移植用。
而周邊血液幹細胞則是指藉由施打白血球生長激素G-CSF),將骨髓中的幹細胞驅動至血液中,再經由血液分離機收集取得之幹細胞的方式。由於與骨髓幹細胞極為相近,現已逐漸取代需要全身麻醉的骨髓抽取手術。

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脂肪幹細胞療效好 「肌萎縮性脊髓側索硬化症」漸動人家屬盼人體試驗
charles201309 在天空部落發表於09:17:36 | Cell Engineering 細胞工程
脂肪幹細胞療效好 「肌萎縮性脊髓側索硬化症」漸動人家屬盼人體試驗
中廣新聞網 – 2014412
(張文祿報導)
 
一名四十歲的手機晶片硬體工程師,兩年前還是一個健康的中年人,現在卻已經無法抱起四歲女兒,言語表達也相當困難,醫師診斷他罹患「肌萎縮性脊髓側索硬化症」,也就是漸凍人症,由於國內醫藥大學跟生技公司合作,以自體脂肪幹細胞應用在漸凍鼠身上,有良好療效,八個月前申請人體試驗,遲遲沒有下文,由於漸凍人發病後,三到四年內會死亡,家屬今天懇求衛福部儘快審核通過,讓郭姓工程師可以延續生命。
郭姓工程師,曾到紐約攻讀電機碩士,平時健康愛運動,但是兩年前,卻開始沒有力氣,容易扭傷,喝水容易嗆到,最後被診斷出罹患漸凍人症,現在已經無法抱起四歲女兒,講話也很困難,一家人陷入愁雲慘霧中,郭先生自知來日無多,願意用僅存的千萬積蓄治療,換取生存的機會,而中國醫藥大學北港附設醫院院長林欣榮跟光麗生醫合作,進行漸凍鼠自體脂肪幹細胞治療,有很大的進展,八個月前申請衛福部人體試驗,但是還沒有核准,郭先生跟太太及媽媽站出來,自願成為試驗對象,郭媽媽說,服貿爭議問題都可以這麼快有進展,希望有關人命的問題,可以加速進行。
林欣榮院長說,自體脂肪幹細胞治療,不必再像以前需要大傷口手術,只需局部麻醉,進入腦部即可,而且可以體外大量培養,不會有免疫排斥。
另外,中國醫藥大學附設醫院與美商永生臍帶血公司也在台中金典酒店舉辦第7屆泛太平洋國際幹細胞及癌症研究研討會,林欣榮院長也在會中發表以幹細胞治療腦中風的臨床試驗成果,移植自體周邊血液幹細胞,可有效改善中風患者的身體失能狀況。

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臨床講義 台灣診斷書
charles201309 在天空部落發表於09:12:52 | Life 人生
臨床講義 台灣診斷書
蔣渭水
 
患者:台灣
 
姓名:台灣島
 
性別︰男
 
年齡:移籍至現住址已二十七歲
 
原籍:中國福建省台灣道
 
現住所:日本帝國台灣總督府
 
職業:世界和平第一關守衛
 
遺傳:明顯地具有黃帝、周公、孔子、孟子等血統
 
素質:為上述聖賢後裔,素質強健,天資聰穎
 
主訴:頭痛、眩暈、腹內飢餓感。最初檢查患者時,以其頭較身大,理應富於思考力,但以二、三常識問題加以詢問,其回答不得要領,可想像患者是個低能兒,頭股雖大,內容空虛,腦髓不充實﹔聞及稍微深入的哲學、數學、科學及世界大勢,便始目暈頭痛
 
既往症:幼年時,身體頗為強壯,頭腦明晰,意志堅強,品行高尚,身手矯健。自入清朝,因受政策毒害,身體逐漸衰弱,意志薄弱,品行低劣,節操低下。轉日本帝國後,接受不晚整的治療,稍見恢復,惟因慢性中毒長達兩百餘年,不易霍然而癒
 
現症:道德頹廢,人心澆漓,物慾旺盛,精神生活貧瘠,風俗醜陋,迷信深固,頑迷不悟,罔顧衛生,智慮淺薄,不知永久大計,只圖眼前小利,墮落怠惰,腐敗、怠慢、虛榮、寡廉鮮恥、四肢倦怠、惰氣滿滿、意氣蕭沉,了無生氣
 
初步診斷︰世界文化的低能兒。
 
原因︰智識的營養不良。
 
經過︰慢性疾病,時日頗長。
 
預斷:因素質純良,若能施以適當療法,尚可迅速治癒。反之,若療法錯誤,延宕時日有病入膏肓之虞。
 
療法:原因療法,即根本治療
 
處方:正規學校教育  最大量
      補習教育    最大量
      幼稚園     最大量    
           讀報社     最大量
 
若能調和上述各劑,迅速服用,可於二十年根治,上有其他特效藥品,此處從略。
 
大正十年(民國十年)十一月一三日
 
主治醫師蔣渭水


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韓國還需要畏懼Chiwan嗎?
charles201309 在天空部落發表於09:01:07 | Life 人生
韓國還需要畏懼Chiwan嗎?
20140413
社論-工商時報 本報訊
 
「反服貿黑箱為始,彰顯台灣意識為終」的太陽花學運英雄式退場,街頭抗爭卻餘波盪漾。這場號稱創造了「一個偉大時代」的新世代運動,為台灣政經發展帶來的究竟是資產還是負債?此時定論尚早,恐怕得花上一個世代來驗證!
由於正反兩造已陷入形同水火的信仰之爭,服貿論證多說無益,因此不妨跳脫內鬥,看看主要對手國南韓的選擇與發展。他山之石可以攻錯,如果我們要以南韓為師,那麼現在的「槍口朝內」,顯然是用錯了方法;如果我們想學不丹標榜幸福感的自給自足式「美好生活」,那麼就要體認目前價廉物美的便利生活,其實是全球化的糖衣毒藥,必須早早切斷捨離。
因為,天下絕無又要馬兒好又要馬兒不吃草的好事,有取有捨才是這個世界運作的硬道理。如果各方對這次學運讓足了利,以致參與者天真的以為對外關係也可以靠佔領與吵鬧而爭得便宜,歸根究柢,還是錯在大人沒教好
以韓國經貿攻略對照台灣服貿抗爭,就不能不提韓國媒體在2009年初新創的關鍵字 Chiwan」,該字將ChinaTaiwan拆組,意在凸顯中台合體將對韓國造成莫大的威脅,旨在喚起韓國上下的憂患意識進而積極應變
Chiwan」形成的時空背景在2008年金融風暴與國共泯恩仇之後。分別以「747」、「636」為治國口號的李明博與馬英九,先後在20082月與5月就任南韓與台灣總統,然而兩人拔高經濟的雄心壯志旋即在下半年遭遇金融風暴突襲。到了該年11月,時任大陸海協會會長陳雲林破天荒訪台,揭開兩岸和解的歷史序幕,緊接著雙方加速洽簽《海峽兩岸經濟合作架構協議》(ECFA),大陸採購團絡繹不絕,「先經後政」的懷柔策略令東亞鄰國大為緊張,深恐海峽組合終將撼動國際政經現勢。
在「Chiwan」的危機感鞭策下,韓國朝野展開了以大陸市場為跳板的全球布局,5年來深耕成果有目共睹。例如三星、LG等大集團不僅在中國各地設廠生產,品牌產品也在當地熱銷;以去年第4季大陸智慧機市占為例,三星仍以19%高居冠軍,技壓陸商聯想(13%)、酷派(11%)、華為(10%),以及美商蘋果(7%)。
尤其在日本因獨島、釣魚台主權問題,先後與南韓、中國鬧翻後,韓國更應用東亞諸國微妙的政經競合,積極拉攏與中國的人民情感。就在太陽花學運吵得沸沸揚揚的期間,以韓劇「來自星星的你」爆紅的男主角「都教授」金秀賢旋風式造訪上海、廣州與北京,所到之處皆引來大量粉絲瘋狂追逐,威力堪比海嘯,連其所屬經紀公司KEYEAST股價也水漲船高,令韓國演藝圈既吃驚又振奮。基於金秀賢與另一火紅男星李敏鎬(韓劇「繼承者們」男主角)在中國大陸的高人氣,南韓軍方日前還特別同意兩人延後入伍,理由正是他們肩負在中國等地推廣韓流的要務。
如今韓流之所以能在中國大陸軟硬通吃,成功絕非偶然。以三星為首的產業界早自1997亞洲金融風暴後即痛定思痛,展開脫胎換骨式轉型。現在的三星,玩自拍行銷,膽子已大到敢在美國總統歐巴馬頭上動土,若沒有堅實的研發與產銷能力支撐,又豈能展現如此氣魄?
南韓流行文化的對外發揚,同樣源於亞洲金融風暴時期,主要推手卻是長期從事反對運動的金大中金大中在1998年就任總統之後,帶著韓國挺過IMF嚴苛金援條件的羞辱、加入世貿組織,並主導文化振興運動,進而開創了韓流風行亞洲的奇蹟。而韓流明星的養成,也絕非整型修容如此簡單,在網路上很容易找到如軍事訓練般嚴格操練的影片,在在顯現「台上十分鐘,台下十年功」的煞費苦心
靠「台下十年功」磨來的成果,還有全球第一的自由貿易協定(FTA)成績單。盧武鉉在2003年接替金大中出任總統,延續其貿易開放政策,制定FTA發展策略藍圖,從此展開漫漫曲折的對外談判路。雖然其間內部波折不斷,但韓國朝野且戰且推進,10年簽定生效的FTA已達9個,涵蓋48個國家。單單今年3月迄今,韓國又宣布與加拿大、澳洲簽署FTA,並與中國啟動第10FTA談判。
反對過度開放的專家學者,或許並不認同南韓的選擇。但開放做為國家級戰略,韓國一以貫之,不因總統換人或政黨輪替而改弦易轍,迄今的發展也證明是利多於弊。
台灣當然大可選擇走自己的路,不必然要與韓國亦步亦趨。但重點是國家發展大計必須有延續性,不可因總統換人或政黨輪替而180度轉彎,否則不僅國人無從適從,更難以獲得外人的慎重對待,他日落得孤島處境,又豈能哭喊冤枉?
時至今日,韓國還需要畏懼Chiwan嗎?答案不言可喻。在反服貿抗爭已演變成獨立性之爭的此際,恐怕台灣自己都不好意思再拿Chiwan來拉抬身價了!

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